Géraldine Delbès

Training openings for students or interns

Areas of expertise

Reproductive Toxicology , Environmental Toxicology , Toxicity of environmental and medical substances on the development of male germ cells , Toxicology

  • INRS Professor

Phone
450 687-5010 poste 8830

Fax
450 686-5501

E-mail
geraldine.delbes@iaf.inrs.ca

Armand-Frappier Santé Biotechnologie Research Centre

531, boulevard des Prairies
Laval (Québec)  H7V 1B7
CANADA

See research centre

Interested research topics

Our central research focus is the investigation of the interface among toxicants, genes, the epigenome and reproductive outcome. Evidence has been accumulating in recent years that the exposure to environmental components and drugs causes reproductive disorders in human population. However, the mechanisms by which such chemicals induce disorders in male fertility and in male germ cells have not been resolved.

Effect of estrogenic compounds on testicular development and adult fertility.

This research will be based on the demonstration that there is a period of increased sensitivity during fetal development of germ cells and that if these cells are exposed at this time there is an increase in reproductive abnormalities and potential the future generations. Epigenetic mechanisms may be involved and their study is one of the research priorities. The research focuses on 4 objectives:

  • Characterization of histone changes in fetal gonads
  • Effect of Low Doses of Estrogen Cocktails on the Development of the Fetal Testicle
  • Effect of estrogen on gene expression, histone changes, and methylation of DNA in gonocytes
  • Long-term effect of estrogen exposure during fetal life on adult fertility

Effect of chemotherapy on the development of male germ cells.

Alkylating agents used for chemotherapy may alter differentiation of germ cells inducing male infertility. Treatments in adults induce a decrease in the production of good quality mature spermatozoa in terms of motility, morphology and genetic integrity. These phenomena may be reversible but no prediction markers are currently available. The only method of preserving fertility for these patients is therefore the cryopreservation of the spermatozoa before treatment. But what about pre-pubertal children, unable to produce sperm? Our research aims to identify fertility biomarkers and evaluate the impact of chemotherapy on the immature testis.

Our research is organized according to 3 axes:

  • Male polymorphism and fertility after chemotherapy
  • Impact of alkylating agents on spermatogonia
  • Study of the quality of the spermatozoa after pre- or post-puberty treatment

Publications

LUSIGNAN MF, LI X, HERRERO B, DELBES G, CHAN PTK. (2018)
Effects of different cryopreservation methods on DNA integrity and sperm chromatin quality in men.
Andrology. 2018 Sep 2

 

TREMBLAY, A., DELBES, G. (2018)
In vitro study of doxorubicin-induced oxidative stress in spermatogonia and immature Sertoli cells
Toxicol Appl Pharmacol. 2018 Jun 1;348:32-42

 

TREMBLAY, A., BEAUD, H., DELBES, G. (2017)
Transgenerational impact of chemotherapy: would the father exposure affect the health of future progeny?
Gynecol Obstet Fertil Senol. 2017 Nov;45(11):609-618

 

RWIGEMERA, A., JOAO, F., DELBES, G. (2017)
Fetal testis organ culture reproduces the dynamics of epigenetic reprogramming in rat gonocytes
Epigenetics Chromatin. 2017 Apr 11;10:19. 

 

BEAUD, H., VAN PELT, A., DELBES, G. (2017)
Doxorubicin and vincristine affect undifferentiated rat spermatogonia.
Reproduction. 2017 Jun;153(6):725-735

 

CHEMEK, M., MIMOUNA, S.B., BOUGHAMMOURA, S., DELBÈS, G., MESSAOUDI, I. (2016)
Protective role of zinc against the toxicity induced by exposure to cadmium during gestation and lactation on testis development.
Reproductive Toxicology, 63, 151-160

 

LASSONDE, G., NASUHOGLU, D., PAN, J. F., GAYE, B., YARGEAU, V. & DELBÈS, G. (2015)
Ozone treatment prevents the toxicity of an environmental mixture of estrogens on rat fetal testicular development,
Reproductive Toxicology, 58, 85-92

 

HABERT, R., MUCZYNSKI, V., GRISIN, T., MOISON, D., MESSIAEN, S., FRYDMAN, R., BENACHI, A., DELBÈS, G., LAMBROT, R., LEHRAIKI, A., N’TUMBA-BYN, T., GUERQUIN, M. J., LEVACHER, C., ROUILLER-FABRE, V. & LIVERA, G. (2014)
Concerns about the widespread use of rodent models for human risk assessments of endocrine disruptors,
Reproduction, 147, 4, R119-29

 

HERRERO, M. B., DELBÈS, G., CHUNG, J. T., SON, W. Y., HOLZER, H., BUCKETT, W. & CHAN, P. (2013)
Case report: the use of annexin V coupled with magnetic activated cell sorting in cryopreserved spermatozoa from a male cancer survivor: healthy twin newborns after two previous ICSI failures,
Journal of Assisted Reproduction and Genetics, 30, 11, 1415-9

 

DELBES, G., HERRERO, M. B., TROEUNG, E. T. & CHAN, P. T. (2013)
The use of complimentary assays to evaluate the enrichment of human sperm quality in asthenoteratozoospermic and teratozoospermic samples processed with Annexin-V magnetic activated cell sorting,
Andrology, 1, 5, 698-706

 

LARCHER, S., DELBÈS, G., ROBAIRE, B. & YARGEAU, V. (2012)
Degradation of 17alpha-ethinylestradiol by ozonation–identification of the by-products and assessment of their estrogenicity and toxicity,
Environment International, 39, 1, 66-72

 

DELBÈS, G., YANAGIYA, A., SONENBERG, N. & ROBAIRE, B. (2012)
PABP interacting protein 2A (PAIP2A) regulates specific key proteins during spermiogenesis in the mouse,
Biology of Reproduction, 86, 3, 95

 

DELBÈS, G., CHAN, D., HALES, B. F., TRASLER, J. M. & ROBAIRE, B. (2012)
Selective induction of glutathione S-transferases in round spermatids from the Brown-Norway rat by the chemotherapeutic regimen for testicular cancer,
Reproductive Toxicology, 36C, 24-32

 

CHAN, D., DELBÈS, G., LANDRY, M., ROBAIRE, B. & TRASLER, J. M. (2012)
Epigenetic alterations in sperm DNA associated with testicular cancer treatment,
Toxicological Sciences, 125, 2, 532-43

 

YANAGIYA, A., DELBÈS, G., SVITKIN, Y. V., ROBAIRE, B. & SONENBERG, N. (2010)
The poly(A)-binding protein partner Paip2a controls translation during late spermiogenesis in mice,
Journal of Clinical Investigation, 120, 9, 3389-400

 

DELBÈS, G., VAISHEVA, F., LUU, T., MARCON, L., HALES, B. F. & ROBAIRE, B. (2010)
Reversibility of the effects of the chemotherapeutic regimen for non-Hodgkin lymphoma, cyclophosphamide, doxorubicin, vincristine, and prednisone, on the male rat reproductive system and progeny outcome,
Reproductive Toxicology, 29, 3, 332-8

 

DELBÈS, G. & SHERINS, R. J. (2010)
Is there a Decline in Sperm Counts in Men?
In: ROBAIRE, B. & CHAN, P. (Eds.) Handbook of Andrology. 2nd Ed ed., The American Society of Andrology.

 

DELBÈS, G., HALES, B. F. & ROBAIRE, B. (2010)
Toxicants and human sperm chromatin integrity,
Molecular Human Reproduction, 16, 1, 14-22

 

DELBÈS, G., CHAN, D., PAKARINEN, P., TRASLER, J. M., HALES, B. F. & ROBAIRE, B. (2009)
Impact of the chemotherapy cocktail used to treat testicular cancer on the gene expression profile of germ cells from male Brown-Norway rats,
Biology of Reproduction, 80, 2, 320-7

 

VAISHEVA, F., DELBÈS, G., HALES, B. F. & ROBAIRE, B. (2007)
Effects of the chemotherapeutic agents for non-Hodgkin lymphoma, cyclophosphamide, doxorubicin, vincristine, and prednisone (CHOP), on the male rat reproductive system and progeny outcome,
Journal of Andrology, 28, 4, 578-87

 

DELBÈS, G., HALES, B. F. & ROBAIRE, B. (2007)
Effects of the chemotherapy cocktail used to treat testicular cancer on sperm chromatin integrity,
Journal of Andrology, 28, 2, 241-9; discussion 250-1

 

DELBÈS, G., DUQUENNE, C., SZENKER, J., TACCOEN, J., HABERT, R. & LEVACHER, C. (2007)
Developmental changes in testicular sensitivity to estrogens throughout fetal and neonatal life,
Toxicological Sciences, 99, 1, 234-43

 

LIVERA, G., DELBÈS, G., PAIRAULT, C., ROUILLER-FABRE, V. & HABERT, R. (2006)
Organotypic culture, a powerful model for studying rat and mouse fetal testis development,
Cell and Tissue Research, 324, 3, 507-21

 

HABERT, R., DELBÈS, G., DUQUENNE, C., LIVERA, G. & LEVACHER, C. (2006)
[Effects of estrogens on the development of the testis during fetal and neonatal life],
Gynecologie, Obstetrique et Fertilite, 34, 10, 970-7

 

DELBÈS, G., LEVACHER, C. & HABERT, R. (2006)
Estrogen effects on fetal and neonatal testicular development,
Reproduction, 132, 4, 527-38

 

DELBÈS, G., LEVACHER, C., DUQUENNE, C., RACINE, C., PAKARINEN, P. & HABERT, R. (2005)
Endogenous estrogens inhibit mouse fetal Leydig cell development via estrogen receptor alpha,
Endocrinology, 146, 5, 2454-61

 

DELBÈS, G., LEVACHER, C., DUQUENNE, C. & HABERT, R. (2005)
[Is fetal testis in danger?],
Médecine sciences : M/S, 21, 12, 1083-8

 

DELBÈS, G., LEVACHER, C., PAIRAULT, C., RACINE, C., DUQUENNE, C., KRUST, A. & HABERT, R. (2004)
Estrogen receptor beta-mediated inhibition of male germ cell line development in mice by endogenous estrogens during perinatal life,
Endocrinology, 145, 7, 3395-403

 

ROUILLER-FABRE, V., LEVACHER, C., PAIRAULT, C., RACINE, C., MOREAU, E., OLASO, R., LIVERA, G., MIGRENNE, S., DELBÈS, G. & HABERT, R. (2003)
Development of the foetal and neonatal testis,
Andrologia, 35, 1, 79-83