Michel Sylvestre

Professeur INRS

Areas of expertise

biocatalysis , Environmental biotechnologies , Enzyme engineering

  • Honorary professor at INRS

Armand-Frappier Santé Biotechnologie Research Centre

531, boulevard des Prairies
Laval (Québec)  H7V 1B7

See research centre


Dhindwal S., Gomez-Gil L., Neau D. B., Pham T. T. M., Sylvestre M., Eltis L. D., Bolin J.T., Kumar P. 2016.The structural basis of the enhanced pollutant-degrading capabilities of an engineered biphenyl dioxygenase. J. Bacteriol. 198(10), 1499-1512


Pham, T. T. M., Pino Rodriguez, Hijiri, M, Sylvestre, M. 2015. Optimizing polychlorinated biphenyl degradation by flavonoid-induced cells of the rhizobacterium Rhodococcus erythropolis U23A. Plos One Published May13 2015, DOI: 101371/journal.pone.0126033.


Pham, T.T.M., Sondossi, M,, Sylvestre, M. 2015. The metabolism of doubly para-substituted hydroxychlorobiphenyls by bacterial biphenyl dioxygenases. Appl. Environ. Microbiol. 81, 4860-4872


Song, L. , Tsang, A. , Sylvestre, M. 2015. Engineering a thermostable fungal GH10 xylanase, importance of N-terminal amino acids. Biotechnol. Bioeng. 112(6), 1081-1091.


Pham, T. T. M. and Sylvestre, M, 2013. Has the bacterial biphenyl catabolic pathway evolved primarily to degrade biphenyl? The diphenylmethane case. J. Bacteriol. 195, 3563-3574.


Sylvestre, M. 2013. Prospects for using combined engineered bacterial enzymes and plant systems to rhizoremediate PCBs. Environmental Microbiology 15(3): 907-915.


Kumar, P., Mohammadi, M., Dhindwal, S., Pham, T.T.M., Bolin, J. T., Sylvestre, M. 2012. Structural insights into the metabolism of 2-chlorodibenzofuran by an evolved biphenyl dioxygenase. Biochem. Biophys. Res. Comm. 421, 757-762. http://www.sciencedirect.com/science/journal/aip/0006291X


Pham T.T. M.,  Tu, Y and Sylvestre, M. 2012.  Remarkable abilities of Pandoraea pnomenusa B356 biphenyl dioxygenase to metabolize simple flavonoids.  Appl. Environ. Microbiol.  78, 3560-3570. http://aem.asm.org/content/78/10/3560.abstract


Viger, J.F, Mhammadi, M, Barriault, D., Sylvestre, M. 2012. Metabolism of chlorobiphenyls by a variant biphenyl dioxygenase exhibiting enhanced activity toward dibenzofuran. Biochem. Biophys. Res. Comm. 419, 362-367 http://www.sciencedirect.com/science/article/pii/S0006291X12002471?v=s5


Toussaint, J-P,  Pham, T.T.M., Barriault, D. and Sylvestre, M. 2012. Plant exudates promote PCB degradation by a rhodococcal rhizobacteria. Appl.Microbiol.Biotechnol. 95, 1589-1603. http://www.springerlink.com/content/l76x2t6521w4802h/


L’Abbée, J. B., Tu, Y. B., Barriault, D., Sylvestre, M. 2011. Insight into the metabolism of 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane (DDT) by biphenyl dioxygenases Arch. Biochem. Biophys. 516, 35-44. http://www.sciencedirect.com/science?_ob=ArticleListURL&_method=list&_ArticleListID=1854440595&_sort=r&_st=13&<??view=c&_acct=C000051292&_version=1&_urlVersion=0&_userid=1072239&md5=0fca32bbcfd4554<?>c156eadae6a2ab280&searchtype=a


Dhindwal, S., D. N. Patil, M. Mohammadi, M. Sylvestre, S. Tomar, and P. Kumar. 2011. Biochemical studies and ligand bound structures of biphenyl dehydrogenase from Pandoraea pnomenusa strain B-356 reveal a basis for broad specificity of the enzyme. J. Biol. Chem. 286, 37011-37022. http://www.jbc.org/content/286/42/37011.abstract?sid=9a7395c8-4e4c-460a-a69a-932c85d04095


Mohammadi, M.,  Viger, J.F., Kumar, P., Barriault, D., Bolin, J. T., Sylvestre, M. 2011. Retuning Rieske-type oxygenase to expand substrate range. J. Biol Chem. 286, 27612-27621. http://www.jbc.org/content/286/31/27612.abstract?sid=9a7395c8-4e4c-460a-a69a-932c85d04095


Kumar, P., Gomez-Gil, L., Mohammadi, M., Sylvestre, M., Eltis, L. D. and Bolin, J. T. 2011. Anaerobic crystallization and initial X-ray diffraction data of biphenyl 2,3-dioxygenase from Burkholderia xenovorans LB400: addition of agarose improved the quality of the crystals. Acta Crystallogr Sect F Struct Biol Cryst Commun 67, 59-63. http://onlinelibrary.wiley.com/doi/10.1107/S1744309110043393/abstract


Kumar, P., Mohammadi, M., Viger, J.F., Barriault, D., Gomez-Gil, L., Eltis, L.D., Bolin, J. T., and Sylvestre, M. 2011. Structural insight into the expanded PCB-degrading abilities of a biphenyl dioxygenase obtained by directed evolution. J. Mol. Biol. 405, 531-547. http://www.sciencedirect.com/science/article/pii/S002228361001209X


Sylvestre, M. and Toussaint, J.P. 2011. Engineering microbial enzymes and plants to promote PCB degradation in soil: Current state of knowledge. In: Microbial Bioremediation of Non-metals: Current Research, Koukkou, A.I. ed. Chapter 8, pp. 177-196. Caister Academic Press, http://www.horizonpress.com/bioremediation


Patil, D. N., Tomar, S., Sylvestre,M. And Kumar, P. 2010. Expression, purification, crystallization and preliminary crystallographic studies of cis-biphenyl-2,3-dihydrodiol-2,3-dehydrogenase from Pandoraea pnomenusa B-356. Acta Crystallogr Sect F Struct Biol Cryst Commun 66, 1517-1520. http://onlinelibrary.wiley.com/doi/10.1107/S1744309110036894/abstract

Sylvestre M, Macek T, Mackova, M. 2009. Transgenic plants to improve rhizoremediation of polychlorinated biphenyls (PCBs). Curr Opin Biotechnol 20, 242-247. http://www.sciencedirect.com/science/article/pii/S0958166909000081


Vézina, J., Barriault, D., and Sylvestre. M. 2008. Diversity of the C-terminal portion of the biphenyl dioxygenase large subunit. J. Mol. Microbiol. Biotechnol.15, 139-151.


Mohammadi, M., Chalavi, V., Surra, M., Laliberté, J.F, Sylvestre, M. 2007. Expression of the bacterial biphenyl-chlorobiphenyl dioxygenase into tobacco plants. Biotechnol Bioeng 97: 496-505. http://onlinelibrary.wiley.com/doi/10.1002/bit.21188/abstract


Vézina, J. and Sylvestre M. 2007. Shuffling soil DNA to change the substrate regiospecificity of the biphenyl dioxygenase. J. Bacteriol 189: 779-788. http://jb.asm.org/content/189/3/779.abstract


Gomez, L, Barriault, D., Sylvestre, M and Eltis L. 2007. Characterization of Biphenyl Dioxygenase of Comamonas testosteroni B-356 as a Potent Polychlorinated Biphenyl-Degrading Enzyme. J. Bacteriol. 189: 5705-5715.


Macková, M., Vrchotová, B., Francová, K., Sylvestre, M., Tomaniová, M., Lovecká, P., Demnerová, K., and Macek, T. 2006. Biotransformation of PCBs by plants and bacteria – consequences of plant-microbe interactions. Europ. J. Soil. Biol. 43:233-241


Mackova, M., Barriault, D., Francová, K., Sylvestre, M., Möder, M., Vrchotová, B., Lovecká, P., Najmanova, J., Demnerova K., Sura, M., Rezek, J., Macek, T. 2006. Phytoremediation of polychlorinated biphenyls. In Phytoremediation and Rhizoremediation, Mackova, M., Dowling, D., Macek, T. eds., Series: Focus on Biotechnology, Vol. 9A Chap. 12. Springer, Life Sciences


L’Abbée, J and. Sylvestre, M. 2005. Metabolism of dibenzofuran and dibenzo-p-dioxin by the biphenyl dioxygenase of Burkholderia xenovorans LB400 and Comamonas testosteroni B-356. Appl. Microbiol. Biotech. 67: 506-514.


Macek, T., Surá, M., Pavliková, D., Francová, K., Scouten, W.H., Szekeres, M., Sylvestre, M. and Macková, M. 2005. Can Tobacco Have Potentially Beneficial Effect to our Health? Z. Naturforschung C 60: 292-299.


Mohammadi, M. and Sylvestre, M. 2005. Resolving the Profile of Metabolites Generated during Oxidation of Dibenzofuran and Chlorodibenzofurans by the Biphenyl Catabolic Pathway Enzymes. Chem Biol 12, 835-846.


Barriault, D., F. Lépine, M. Mohammadi, S. Milot, N. Leberre, and Sylvestre, M. 2004.  Revisiting the regiospecificity of Burkholderia xenovorans LB400 biphenyl dioxygenase toward 2,2′-dichlorobiphenyl and 2,3,2′,3′-tetrachlorobiphenyl. J. Biol. Chem. 279(46): 47489-47496.


Barriault, D., and Sylvestre, M. 2004.  Evolution of the biphenyl dioxygenase BphA from Burkholderia xenovorans LB400 by random mutagenesis of multiple sites in region III. J. Biol. Chem. 279(46): 47480-47488.

Song, L. , Tsang, A. , Sylvestre, M. 2015. Engineering a thermostable fungal GH10 xylanase, importance of N-terminal amino acids. Biotechnol. Bioeng.  01/2015; DOI: 10.1002/bit.25533