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Michel Sylvestre

Professeur INRS

Areas of expertise

Environmental biotechnologies , biocatalysis , Enzyme engineering

  • Honorary Professor

Armand-Frappier Santé Biotechnologie Research Centre

531 des Prairies Blvd.
Laval, Quebec  H7V 1B7

See the research centre


Dhindwal S., Gomez-Gil L., Neau D. B., Pham T. T. M., Sylvestre M., Eltis L. D., Bolin J.T., Kumar P. 2016.The structural basis of the enhanced pollutant-degrading capabilities of an engineered biphenyl dioxygenase. J. Bacteriol. 198(10), 1499-1512


Pham, T. T. M., Pino Rodriguez, Hijiri, M, Sylvestre, M. 2015. Optimizing polychlorinated biphenyl degradation by flavonoid-induced cells of the rhizobacterium Rhodococcus erythropolis U23A. Plos One Published May13 2015, DOI: 101371/journal.pone.0126033.


Pham, T.T.M., Sondossi, M,, Sylvestre, M. 2015. The metabolism of doubly para-substituted hydroxychlorobiphenyls by bacterial biphenyl dioxygenases. Appl. Environ. Microbiol. 81, 4860-4872


Song, L. , Tsang, A. , Sylvestre, M. 2015. Engineering a thermostable fungal GH10 xylanase, importance of N-terminal amino acids. Biotechnol. Bioeng. 112(6), 1081-1091.


Pham, T. T. M. and Sylvestre, M, 2013. Has the bacterial biphenyl catabolic pathway evolved primarily to degrade biphenyl? The diphenylmethane case. J. Bacteriol. 195, 3563-3574.


Sylvestre, M. 2013. Prospects for using combined engineered bacterial enzymes and plant systems to rhizoremediate PCBs. Environmental Microbiology 15(3): 907-915.


Kumar, P., Mohammadi, M., Dhindwal, S., Pham, T.T.M., Bolin, J. T., Sylvestre, M. 2012. Structural insights into the metabolism of 2-chlorodibenzofuran by an evolved biphenyl dioxygenase. Biochem. Biophys. Res. Comm. 421, 757-762.


Pham T.T. M.,  Tu, Y and Sylvestre, M. 2012.  Remarkable abilities of Pandoraea pnomenusa B356 biphenyl dioxygenase to metabolize simple flavonoids.  Appl. Environ. Microbiol.  78, 3560-3570.


Viger, J.F, Mhammadi, M, Barriault, D., Sylvestre, M. 2012. Metabolism of chlorobiphenyls by a variant biphenyl dioxygenase exhibiting enhanced activity toward dibenzofuran. Biochem. Biophys. Res. Comm. 419, 362-367


Toussaint, J-P,  Pham, T.T.M., Barriault, D. and Sylvestre, M. 2012. Plant exudates promote PCB degradation by a rhodococcal rhizobacteria. Appl.Microbiol.Biotechnol. 95, 1589-1603.


L’Abbée, J. B., Tu, Y. B., Barriault, D., Sylvestre, M. 2011. Insight into the metabolism of 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane (DDT) by biphenyl dioxygenases Arch. Biochem. Biophys. 516, 35-44.<??view=c&_acct=C000051292&_version=1&_urlVersion=0&_userid=1072239&md5=0fca32bbcfd4554<?>c156eadae6a2ab280&searchtype=a


Dhindwal, S., D. N. Patil, M. Mohammadi, M. Sylvestre, S. Tomar, and P. Kumar. 2011. Biochemical studies and ligand bound structures of biphenyl dehydrogenase from Pandoraea pnomenusa strain B-356 reveal a basis for broad specificity of the enzyme. J. Biol. Chem. 286, 37011-37022.


Mohammadi, M.,  Viger, J.F., Kumar, P., Barriault, D., Bolin, J. T., Sylvestre, M. 2011. Retuning Rieske-type oxygenase to expand substrate range. J. Biol Chem. 286, 27612-27621.


Kumar, P., Gomez-Gil, L., Mohammadi, M., Sylvestre, M., Eltis, L. D. and Bolin, J. T. 2011. Anaerobic crystallization and initial X-ray diffraction data of biphenyl 2,3-dioxygenase from Burkholderia xenovorans LB400: addition of agarose improved the quality of the crystals. Acta Crystallogr Sect F Struct Biol Cryst Commun 67, 59-63.


Kumar, P., Mohammadi, M., Viger, J.F., Barriault, D., Gomez-Gil, L., Eltis, L.D., Bolin, J. T., and Sylvestre, M. 2011. Structural insight into the expanded PCB-degrading abilities of a biphenyl dioxygenase obtained by directed evolution. J. Mol. Biol. 405, 531-547.


Sylvestre, M. and Toussaint, J.P. 2011. Engineering microbial enzymes and plants to promote PCB degradation in soil: Current state of knowledge. In: Microbial Bioremediation of Non-metals: Current Research, Koukkou, A.I. ed. Chapter 8, pp. 177-196. Caister Academic Press,


Patil, D. N., Tomar, S., Sylvestre,M. And Kumar, P. 2010. Expression, purification, crystallization and preliminary crystallographic studies of cis-biphenyl-2,3-dihydrodiol-2,3-dehydrogenase from Pandoraea pnomenusa B-356. Acta Crystallogr Sect F Struct Biol Cryst Commun 66, 1517-1520.

Sylvestre M, Macek T, Mackova, M. 2009. Transgenic plants to improve rhizoremediation of polychlorinated biphenyls (PCBs). Curr Opin Biotechnol 20, 242-247.


Vézina, J., Barriault, D., and Sylvestre. M. 2008. Diversity of the C-terminal portion of the biphenyl dioxygenase large subunit. J. Mol. Microbiol. Biotechnol.15, 139-151.


Mohammadi, M., Chalavi, V., Surra, M., Laliberté, J.F, Sylvestre, M. 2007. Expression of the bacterial biphenyl-chlorobiphenyl dioxygenase into tobacco plants. Biotechnol Bioeng 97: 496-505.


Vézina, J. and Sylvestre M. 2007. Shuffling soil DNA to change the substrate regiospecificity of the biphenyl dioxygenase. J. Bacteriol 189: 779-788.


Gomez, L, Barriault, D., Sylvestre, M and Eltis L. 2007. Characterization of Biphenyl Dioxygenase of Comamonas testosteroni B-356 as a Potent Polychlorinated Biphenyl-Degrading Enzyme. J. Bacteriol. 189: 5705-5715.


Macková, M., Vrchotová, B., Francová, K., Sylvestre, M., Tomaniová, M., Lovecká, P., Demnerová, K., and Macek, T. 2006. Biotransformation of PCBs by plants and bacteria – consequences of plant-microbe interactions. Europ. J. Soil. Biol. 43:233-241


Mackova, M., Barriault, D., Francová, K., Sylvestre, M., Möder, M., Vrchotová, B., Lovecká, P., Najmanova, J., Demnerova K., Sura, M., Rezek, J., Macek, T. 2006. Phytoremediation of polychlorinated biphenyls. In Phytoremediation and Rhizoremediation, Mackova, M., Dowling, D., Macek, T. eds., Series: Focus on Biotechnology, Vol. 9A Chap. 12. Springer, Life Sciences


L’Abbée, J and. Sylvestre, M. 2005. Metabolism of dibenzofuran and dibenzo-p-dioxin by the biphenyl dioxygenase of Burkholderia xenovorans LB400 and Comamonas testosteroni B-356. Appl. Microbiol. Biotech. 67: 506-514.


Macek, T., Surá, M., Pavliková, D., Francová, K., Scouten, W.H., Szekeres, M., Sylvestre, M. and Macková, M. 2005. Can Tobacco Have Potentially Beneficial Effect to our Health? Z. Naturforschung C 60: 292-299.


Mohammadi, M. and Sylvestre, M. 2005. Resolving the Profile of Metabolites Generated during Oxidation of Dibenzofuran and Chlorodibenzofurans by the Biphenyl Catabolic Pathway Enzymes. Chem Biol 12, 835-846.


Barriault, D., F. Lépine, M. Mohammadi, S. Milot, N. Leberre, and Sylvestre, M. 2004.  Revisiting the regiospecificity of Burkholderia xenovorans LB400 biphenyl dioxygenase toward 2,2′-dichlorobiphenyl and 2,3,2′,3′-tetrachlorobiphenyl. J. Biol. Chem. 279(46): 47489-47496.


Barriault, D., and Sylvestre, M. 2004.  Evolution of the biphenyl dioxygenase BphA from Burkholderia xenovorans LB400 by random mutagenesis of multiple sites in region III. J. Biol. Chem. 279(46): 47480-47488.

Song, L. , Tsang, A. , Sylvestre, M. 2015. Engineering a thermostable fungal GH10 xylanase, importance of N-terminal amino acids. Biotechnol. Bioeng.  01/2015; DOI: 10.1002/bit.25533