Charles M. Dozois

Professeur INRS

Areas of expertise

Identification and characterization of bacterial genes affecting humans, poultry and pigs , Microbiology and immunology

  • INRS Professor

Phone
450 687-5010

Fax
450 686-5501

E-mail
charles.dozois@iaf.inrs.ca

Armand-Frappier Santé Biotechnologie Research Centre

531, boulevard des Prairies
Laval, Québec  H7V 1B7
CANADA

See research centre

Interested research topics

  • Bacterial gene regulation and expression during infection
  • Vaccine development
  • Enterobacterial pathogens causing diseases of importance to human and animal health
  • Microbial metal transport in pathogenic bacteria including Escherichia coliSalmonella, and Klebsiella
  • Bacterial comparative and functional genomics

International Innovation, octobre 2013, page 48, INRS-Institut Armand-Frappier Research Centre

Dr. Charles M. Dozois received a B.Sc. (Agr.) in Biological Sciences from the Faculty of Agriculture of McGill University (1990), an M.Sc. (1993) in Veterinary Microbiology from the Faculty of Veterinary Medicine of the University of Montreal, and a Ph. D.(1995) in Microbiology-Immunology from the Faculty of Medicine of the University of Montreal.

Following work as a postdoctoral fellow in immunology at INRA (Toulouse, France) and a research associateship at the Dept. of Biology of Washington University (Saint-Louis, MO, USA), he joined the INRS – Institut Armand-Frappier as a professor in 2001.

His research centres on further understanding the mechanisms of host-pathogen interactions. In particular, Dr. Dozois is interested in the identification and characterisation of bacterial genes that are expressed in vivo during infection of the host and the discovery of new microbial systems that contribute to pathogenesis.

Publications

ZEBIAN, N., MERKX-JACQUES, A., PITTOCK, P. P., HOULE, S., DOZOIS, C. M., LAJOIE, G. A. & CREUZENET, C. (2015)
Comprehensive analysis of flagellin glycosylation in Campylobacter jejuni NCTC 11168 reveals incorporation of legionaminic acid and its importance for host colonization,
Glycobiology.

CYOIA, P. S., RODRIGUES, G. R., NISHIO, E. K., MEDEIROS, L. P., KOGA, V. L., PEREIRA, A. P. D., VESPERO, E. C., HOULE, S., DOZOIS, C. M., NAKAZATO, G. & KOBAYASHI, R. K. T. (2015)
Presence of virulence genes and pathogenicity islands in extraintestinal pathogenic escherichia coli isolates from Brazil,
Journal of Infection in Developing Countries, 9, 10, 1068-1075.

MURASE, K., MARTIN, P., PORCHERON, G., HOULE, S., HELLOIN, E., PENARY, M., NOUGAYREDE, J. P., DOZOIS, C. M., HAYASHI, T. & OSWALD, E. (2015)
HlyF Produced by Extraintestinal Pathogenic Escherichia coli Is a Virulence Factor That Regulates Outer Membrane Vesicle Biogenesis,
Journal of Infectious Diseases.

CAZA, M., GARENAUX, A., LÉPINE, F. & DOZOIS, C. M. (2015)
Catecholate siderophore esterases Fes, IroD and IroE are required for salmochelins secretion following utilization, but only IroD contributes to virulence of extra-intestinal pathogenic Escherichia coli,
Molecular Microbiology, 97, 4, 717-32.

PORCHERON, G. & DOZOIS, C. M. (2015)
Interplay between iron homeostasis and virulence: Fur and RyhB as major regulators of bacterial pathogenicity,
Veterinary Microbiology, 179, 1-2, 2-14.

WONG, A., LANGE, D., HOULE, S., ARBATSKY, N. P., VALVANO, M. A., KNIREL, Y. A., DOZOIS, C. M. & CREUZENET, C. (2015)
Role of capsular modified heptose in the virulence of Campylobacter jejuni,
Molecular Microbiology, 96, 6, 1136-1158.

CHEKABAB, S. M., HAREL, J. & DOZOIS, C. M. (2014)
Interplay between genetic regulation of phosphate homeostasis and bacterial virulence,
Virulence, 5, 8, 786-793.

MALUTA, R. P., GATTI, M. S. V., JOAZEIRO, P. P., DE PAIVA, J. B., ROJAS, T. C. G., SILVEIRA, F., HOULE, S., KOBAYASHI, R. K. T., DOZOIS, C. M. & DIAS DA SILVEIRA, W. (2014)
Avian extraintestinal escherichia coli exhibits enterotoxigenic-like activity in the in vivo rabbit ligated ileal loop assay,
Foodborne Pathogens and Disease, 11, 6, 484-489.

CHEKABAB, S. M., JUBELIN, G., DOZOIS, C. M. & HAREL, J. (2014)
PhoB Activates Escherichia coli O157:H7 Virulence Factors in Response to Inorganic Phosphate Limitation,
PLoS ONE, 9, 4, e94285.

PORCHERON, G., GARENAUX, A., PROULX, J., SABRI, M. & DOZOIS, C. M. (2013)
Iron, copper, zinc, and manganese transport and regulation in pathogenic Enterobacteria: correlations between strains, site of infection and the relative importance of the different metal transport systems for virulence,
Frontiers in cellular and infection microbiology, 3, 90.

LECLERC, J. M., DOZOIS, C. M. & DAIGLE, F. (2013)
Role of the Salmonella enterica serovar Typhi Fur regulator and small RNAs RfrA and RfrB in iron homeostasis and interaction with host cells,
Microbiology (United Kingdom), 159, Pt 3, 591-602.

GARÉNAUX, A., HOULE, S., FOLCH, B., DALLAIRE, G., TRUESDELL, M., LÉPINE, F., DOUCET, N. & DOZOIS, C. M. (2013)
Avian lipocalin expression in chickens following Escherichia coli infection and inhibition of avian pathogenic Escherichia coli growth by Ex-FABP,
Veterinary Immunology and Immunopathology, 152, 1-2, 156-67.

CHEKABAB, S. M., PAQUIN-VEILLETTE, J., DOZOIS, C. M. & HAREL, J. (2013)
The ecological habitat and transmission of Escherichia coli O157:H7,
FEMS Microbiology Letters, 341, 1, 1-12.

CHEKABAB, S. M., DAIGLE, F., CHARETTE, S. J., DOZOIS, C. M. & HAREL, J. (2013)
Shiga toxins decrease enterohaemorrhagic Escherichia coli survival within Acanthamoeba castellanii,
FEMS Microbiology Letters.

CREPIN, S., PORCHERON, G., HAREL, J. & DOZOIS, C. M. (2012)
The fate of not sensing phosphate: downregulation of type 1 fimbriae through activation of the Pho regulon reduces E. coli urinary tract virulence,
Virulence, 3, 7, 596-8.

GARENAUX, A. & DOZOIS, C. M. (2012)
Metals: Ironing out copper toxicity
Nature Chemical Biology , 8, 8, 680-1

CRÉPIN, S., HOULE, S., CHARBONNEAU, M.-È., MOUREZ, M., HAREL, J. & DOZOIS, C. M. (2012)
Decreased expression of type 1 fimbriae by a pst mutant of uropathogenic Escherichia coli reduces urinary tract infection
Infection and Immunity , 80, 8, 2802-2815.

CRÉPIN, S., HAREL, J. & DOZOIS, C. M. (2012)
Chromosomal complementation using tn7 transposon vectors in enterobacteriaceae
Applied and Environmental Microbiology , 78, 17, 6001-8.

COTÉ, J. P., BERTHIAUME, F., HOULE, S., FAIRBROTHER, J. M., DOZOIS, C. M. & MOUREZ, M. (2012)
Identification and Mechanism of Evolution of New Alleles Coding for the AIDA-I Autotransporter of Porcine Pathogenic Escherichia coli
Applied and Environmental Microbiology , 78, 13, 4597-4605.

CHARBONNEAU, M.-È., CÔTÉ, J.-P., HAURAT, M. F., REIZ, B., CRÉPIN, S., BERTHIAUME, F., DOZOIS, C. M., FELDMAN, M. F. & MOUREZ, M. (2012)
A structural motif is the recognition site for a new family of bacterial protein O-glycosyltransferases
Molecular Microbiology , 83, 5., 894-907.

GARENAUX, A., CAZA, M. & DOZOIS, C. M. (2011)
The Ins and Outs of siderophore mediated iron uptake by extra-intestinal pathogenic Escherichia coli
Veterinary Microbiology , 153, 1-2, 89-98.

DOZOIS, C. M., GARENAUX, A., HOULE, S., DALLAIRE, G., TRUESDELL, M. & POIRIER, K. (2011)
Avian lipocalin expression following E. coli infection and inhibition of bacterial growth in vitro .
Frontiers in Immunology .

CRÉPIN, S., CHEKABAB, S.-M., LE BIHAN, G., BERTRAND, N., DOZOIS, C. M. & HAREL, J. (2011)
The Pho regulon and the pathogenesis of Escherichia coli
Veterinary Microbiology , 153, 1-2, 82-88.

CAZA, M., LÉPINE, F. & DOZOIS, C. M. (2011)
Secretion, but not overall synthesis, of catecholate siderophores contributes to virulence of extraintestinal pathogenic Escherichia coli
Molecular Microbiology , 80, 1, 266-82.

BÉLANGER, L., GARENAUX, A., HAREL, J., BOULIANNE, M., NADEAU, E. & DOZOIS, C. M. (2011)
Escherichia coli from animal reservoirs as a potential source of human extraintestinal pathogenic E-coli
FEMS Immunology and Medical Microbiology , 62, 1, 1-10.

BACHMAN, M. A., OYLER, J. E., BURNS, S. H., CAZA, M., LÉPINE, F., DOZOIS, C. M. & WEISER, J. N. (2011)
Klebsiella pneumoniae Yersiniabactin Promotes Respiratory Tract Infection through Evasion of Lipocalin 2
Infection and Immunity , 79, 8, 3309-3316.

VINCENT, C., BOERLIN, P., DAIGNAULT, D., DOZOIS, C. M., DUTIL, L., GALANAKIS, C., REID-SMITH, R. J., TELLIER, P. P., TELLIS, P. A., ZIEBELL, K. & MANGES, A. R. (2010)
Food reservoir for Escherichia coli causing urinary tract infections
Emerging Infectious Diseases , 16, 1, 88-95.

SALVAIL, H., LANTHIER-BOURBONNAIS, P., SOBOTA, J. M., CAZA, M., BENJAMIN, J. A. M., MENDIETA, M. E. S., LÉPINE, F., DOZOIS, C. M., IMLAY, J. & MASSÉ, E. (2010)
A small RNA promotes siderophore production through transcriptional and metabolic remodeling
Proceedings of the National Academy of Sciences of the United States of America , 107, 34, 15223-15228.

BERTRAND, N., HOULE, S., LEBIHAN, G., POIRIER, E., DOZOIS, C. M. & HAREL, J. (2010)
Increased Pho Regulon Activation Correlates with Decreased Virulence of an Avian Pathogenic Escherichia coli O78 Strain
Infection and Immunity , 78, 12, 5324-5331.

SABRI, M., HOULE, S. & DOZOIS, C. M. (2009)
Roles of the extraintestinal pathogenic escherichia coli ZnuACB and ZupT Zinc Transporters during Urinary Tract Infection
Infection and Immunity , 77, 3, 1155-1164.

SABRI, M., CAZA, M., PROULX, J., LYMBEROPOULOS, M. H., BREE, A., MOULIN-SCHOULEUR, M., CURTISS, R., III & DOZOIS, C. M. (2008)
Contribution of the SitABCD, MntH, and FeoB metal transporters to the virulence of avian pathogenic Escherichia coli O78 strain chi 7122
Infection and Immunity , 76, 2, 601-611.

CRÉPIN, S., LAMARCHE, M. G., GARNEAU, P., SÉGUIN, J., PROULX, J., DOZOIS, C. M. & HAREL, J. (2008)
Genome-wide transcriptional response of an avian pathogenic Escherichia coli (APEC) pst mutant
BMC Genomics , 9, 568.

CAZA, M., LÉPINE, F., MILOT, S. & DOZOIS, C. M. (2008)
Specific roles of the iroBCDEN genes in virulence of an avian pathogenic Eschetichia coli O78 strain and in production of salmochelins
Infection and Immunity , 76, 8, 3539-3549.

RESTIERI, C., GARRISS, G., LOCAS, M. C. & DOZOIS, C. M. (2007)
Autotransporter-encoding sequences are phylogenetically distributed among Escherichia coli clinical isolates and reference strains
Applied and Environmental Microbiology , 73, 5, 1553-1562.

FOREST, C., FAUCHER, S. P., POIRIER, K., HOULE, S., DOZOIS, C. M. & DAIGLE, F. (2007)
Contribution of the stg fimbrial operon of Salmonella entelica serovar typhi during interaction with human cells
Infection and Immunity , 75, 11, 5264-5271.

VIJAYAKUMAR, S., MERKX-JACQUES, A., RATNAYAKE, D. B., GRYSKI, I., OBHI, R. K., HOULE, S., DOZOIS, C. M. & CREUZENET, C. (2006)
Cj1121c, a novel UDP-4-keto-6-deoxy-GlcNAc C-4 aminotransferase essential for protein glycosylation and virulence in Campylobacter jejuni
Journal of Biological Chemistry , 281, 38, 27733-27743.

SABRI, M., LÉVEILLÉ, S. & DOZOIS, C. M. (2006)
A SitABCD homologue from an avian pathogenic Escherichia coli strain mediates transport of iron and manganese and resistance to hydrogen peroxide
Microbiology , 152, Pt 3, 745-58.

LYMBEROPOULOS, M. H., HOULE, S., DAIGLE, F., LÉVEILLÉ, S., BRÉE, A., MOULIN-SCHOULEUR, M., JOHNSON, J. R. & DOZOIS, C. M. (2006)
Characterization of Stg fimbriae from an avian pathogenic Eschetichia coli O78 : K80 strain and assessment of their contribution to colonization of the chicken respiratory tract
Journal of Bacteriology , 188, 18, 6449-6459.

LÉVEILLÉ, S., CAZA, M., JOHNSON, J. R., CLABOTS, C., SABRI, M. & DOZOIS, C. M. (2006)
Iha from an Escherichia coli urinary tract infection outbreak clonal group A strain is expressed in vivo in the mouse urinary tract and functions as a catecholate siderophore receptor
Infection and Immunity , 74, 6, 3427-3436.

FAUCHER, S. P., PORWOLLIK, S., DOZOIS, C. M., MCCLELLAND, M. & DAIGLE, F. (2006)
Transcriptome of Salmonella enterica serovar Typhi within macrophages revealed through the selective capture of transcribed sequences
Proceedings of the National Academy of Sciences of the United States of America , 103, 6, 1906-1911.

FAUCHER, S. P., PORWOLLIK, S., DOZOIS, C. M., MCCLELLAND, M. & DAIGLE, F. (2006)
[Salmonella transcriptome during macrophage infection]
Médecine sciences : M/S , 22, 10, 792-3.

LAMARCHE, M. G., DOZOIS, C. M., DAIGLE, F., CAZA, M., CURTISS, R., III, DUBREUIL, J. D. & HAREL, J. (2005)
Inactivation of the Pst system reduces the virulence of an avian pathogenic Escherichia coli O78 strain
Infection and Immunity , 73, 7, 4138-4145.

MELLATA, M., DHO-MOULIN, M., DOZOIS, C. M., CURTISS, R., III, LEHOUX, B. & FAIRBROTHER, J. M. (2003)
Role of avian pathogenic Escherichia coli virulence factors in bacterial interaction with chicken heterophils and macrophages
Infection and Immunity , 71, 1, 494-503.

MELLATA, M., DHO-MOULIN, M., DOZOIS, C. M., CURTISS III, R., BROWN, P. K., ARNÉ, P., BRÉE, A., DESAUTELS, C. & FAIRBROTHER, J. M. (2003)
Role of virulence factors in resistance of avian pathogenic Escherichia coli to serum and in pathogenicity
Infection and Immunity , 71, 1, 536-540.

DOZOIS, C. M., DAIGLE, F. & CURTISS, R., 3RD (2003)
Identification of pathogen-specific and conserved genes expressed in vivo by an avian pathogenic Escherichia coli strain
Proceedings of the National Academy of Sciences of the United States of America , 100, 1, 247-52.

OTTO, B. R., VAN DOOREN, S. J., DOZOIS, C. M., LUIRINK, J. & OUDEGA, B. (2002)
Escherichia coli hemoglobin protease autotransporter contributes to synergistic abscess formation and heme-dependent growth of Bacteroides fragilis
Infection and Immunity , 70, 1, 5-10.

KANG, H. Y., DOZOIS, C. M., TINGE, S. A., LEE, T. H. & CURTISS, R., 3RD (2002)
Transduction-mediated transfer of unmarked deletion and point mutations through use of counterselectable suicide vectors
Journal of Bacteriology , 184, 1, 307-12.

OSWALD, I. P., DOZOIS, C. M., BARLAGNE, R., FOURNOUT, S., JOHANSEN, M. V. & BOGH, H. O. (2001)
Cytokine mRNA expression in pigs infected with Schistosoma japonicum
Parasitology , 122, Pt 3, 299-307.

BROWN, P. K., DOZOIS, C. M., NICKERSON, C. A., ZUPPARDO, A., TERLONGE, J. & CURTISS, R., 3RD (2001)
MlrA, a novel regulator of curli (AgF) and extracellular matrix synthesis by Escherichia coli and Salmonella enterica serovar Typhimurium
Molecular Microbiology , 41, 2, 349-63.

FOURNOUT, S., DOZOIS, C. M., YERLE, M., PINTON, P., FAIRBROTHER, J. M., OSWALD, E. & OSWALD, I. P. (2000)
Cloning, chromosomal location, and tissue expression of the gene for pig interleukin-18
Immunogenetics , 51, 4-5, 358-65.

FOURNOUT, S., DOZOIS, C. M., ODIN, M., DESAUTELS, C., PERES, S., HERAULT, F., DAIGLE, F., SEGAFREDO, C., LAFFITTE, J., OSWALD, E., FAIRBROTHER, J. M. & OSWALD, I. P. (2000)
Lack of a role of cytotoxic necrotizing factor 1 toxin from Escherichia coli in bacterial pathogenicity and host cytokine response in infected germfree piglets
Infection and Immunity , 68, 2, 839-47.

DOZOIS, C. M., DHO-MOULIN, M., BREE, A., FAIRBROTHER, J. M., DESAUTELS, C. & CURTISS, R., 3RD (2000)
Relationship between the Tsh autotransporter and pathogenicity of avian Escherichia coli and localization and analysis of the Tsh genetic region
Infection and Immunity , 68, 7, 4145-54.

OSWALD, I. P., DOZOIS, C. M., FOURNOUT, S., PETIT, J. F. & LEMAIRE, G. (1999)
Tumor necrosis factor is required for the priming of peritoneal macrophages by trehalose dimycolate
European Cytokine Network , 10, 4, 533-40.

DOZOIS, C. M. & CURTISS, R., 3RD (1999)
Pathogenic diversity of Escherichia coli and the emergence of ‘exotic’ islands in the gene stream
Veterinary Research , 30, 2-3, 157-79.

POURBAKHSH, S. A., BOULIANNE, M., MARTINEAU-DOIZE, B., DOZOIS, C. M., DESAUTELS, C. & FAIRBROTHER, J. M. (1997)
Dynamics of Escherichia coil infection in experimentally inoculated chickens
Avian Diseases , 41, 1, 221-33.

OSWALD, I. P., DOZOIS, C. M., PETIT, J. F. & LEMAIRE, G. (1997)
Interleukin-12 synthesis is a required step in trehalose dimycolate-induced activation of mouse peritoneal macrophages
Infection and Immunity , 65, 4, 1364-9.

DOZOIS, C. M., OSWALD, E., GAUTIER, N., SERTHELON, J. P., FAIRBROTHER, J. M. & OSWALD, I. P. (1997)
A reverse transcription-polymerase chain reaction method to analyze porcine cytokine gene expression
Veterinary Immunology and Immunopathology , 58, 3-4, 287-300.

DOZOIS, C. M., CLEMENT, S., DESAUTELS, C., OSWALD, E. & FAIRBROTHER, J. M. (1997)
Expression of P, S, and F1C adhesins by cytotoxic necrotizing factor 1-producing Escherichia coli from septicemic and diarrheic pigs
FEMS Microbiology Letters , 152, 2, 307-12.

DAIGLE, F., DOZOIS, C. M., JACQUES, M. & HAREL, J. (1997)
Mutations in the f165(1)A and f165(1)E fimbrial genes and regulation of their expression in an Escherichia coli strain causing septicemia in pigs
Microbial Pathogenesis , 22, 4, 247-52.

DOZOIS, C. M., HAREL, J. & FAIRBROTHER, J. M. (1996)
P-fimbriae-producing septicaemic Escherichia coli from poultry possess fel-related gene clusters whereas pap-hybridizing P-fimbriae-negative strains have partial or divergent P fimbrial gene clusters
Microbiology , 142 ( Pt 10), 2759-66.

DOZOIS, C. M., POURBAKHSH, S. A. & FAIRBROTHER, J. M. (1995)
Expression of P and type 1 (F1) fimbriae in pathogenic Escherichia coli from poultry
Veterinary Microbiology , 45, 4, 297-309.

DOZOIS, C. M., CHANTELOUP, N., DHO-MOULIN, M., BREE, A., DESAUTELS, C. & FAIRBROTHER, J. M. (1994)
Bacterial colonization and in vivo expression of F1 (type 1) fimbrial antigens in chickens experimentally infected with pathogenic Escherichia coli
Avian Diseases , 38, 2, 231-9.